Dimerization of MLL fusion proteins and FLT3 activation synergize to induce multiple-lineage leukemogenesis
J. Clin. Invest. Ryoichi Ono, et al. 115:919 doi:10.1172/JCI22725 [
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Figure 2Characterization of the cells immortalized by MLL-SEPT6. (
A) Schematic representation of pMXs-neo-MLL-SEPT6. The restriction endonuclease sites and the Neo probe used in the Southern blot analysis are indicated by vertical arrows and a thick horizontal line, respectively. The primers used are indicated by horizontal arrows. LTR, long-terminal repeat. (
B) Immunophenotype of the cells immortalized by MLL-SEPT6. Shadow profiles represent FACS staining obtained with Abs specific for the indicated cell surface antigens. Green lines represent staining obtained with isotype control Abs. (
C) Southern blot analysis to detect clonality (lane 1) and integration (lane 2). Genomic DNA extracted from the cells immortalized by MLL-SEPT6 was digested with BamHI (lane 1) or NheI (lane 2), and hybridized with the Neo probe. Oligoclonal bands of proviral integration sites and a single band of the intact proviral DNA are indicated by arrows and an arrowhead, respectively. (
D) Expression of MLL-SEPT6 fusion transcripts by RT-PCR. M, 1-kb DNA ladder (New England Biolabs Inc.); lane 1, cells immortalized by MLL-SEPT6; lane 2, negative control; lane 3, positive control (pMXs-neo-MLL-SEPT6).
