Staphylococcus aureus Panton-Valentine leukocidin directly targets mitochondria and induces Bax-independent apoptosis
of human neutrophils
J. Clin. Invest. Anne-Laure Genestier, et al. 115:3117 doi:10.1172/JCI22684 [
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Figure 7rPVL induces the release of the apoptogenic proteins cytochrome
c and Smac/DIABLO from isolated mitochondria. Mitochondria from rat liver were treated with increasing concentrations of rPVL (
A), rLukS (
B), or Bax α (
C) for 1 hour at 30°C. Mitochondrial proteins were then separated by SDS-PAGE and analyzed by Western blotting using antibodies directed against cytochrome
c, Smac/DIABLO, and cytochrome oxydase subunit IV (COx IV). Data are from 1 of 2 independent experiments that gave similar results.
