Reduced abundance of syntaxin4 in Munc18c^−/− adipocytes. (A) Northern blot analysis of mRNAs for SNARE proteins. Total RNA extracted from Munc18c^+/+, Munc18c^+/–, or Munc18c^–/– adipocytes was analyzed for hybridization with probes specific for Munc18c, syntaxin4, SNAP23, VAMP2, or Munc18b mRNAs. The region of the ethidium bromide–stained gel containing 28S ribosomal RNA (rRNA) is also shown. (B) Immunoblot analysis of SNARE proteins. Lysates prepared from adipocytes of each genotype were subjected to immunoblot analysis with antibodies specific for the indicated proteins.