Ca_V2.3 calcium channels control second-phase insulin release
J. Clin. Invest. Xingjun Jing, et al. 115:146 doi:10.1172/JCI22518 [Go to this article.]

Figure 2
Effects of CaV2.3 ablation on single-cell exocytosis in islet cells. (A) Exocytosis evoked by trains of 10 depolarizations (V) and monitored as increases in cell capacitance (ØC) in WT CaV2.3^+/+ (black) and CaV2.3^–/– (gray) β cells. (B) Average total increase in capacitance evoked by the trains (ØC_TOT). Data are mean values ± SEM in 6 WT (black bars) and 7 CaV2.3^–/– (gray bars) β cells. *P < 0.05. (C) ØC evoked by intracellular dialysis of a Ca²⁺-containing patch electrode solution (free [Ca²⁺]_i, approximately 1.5 μM) in WT (black) and CaV2.3^–/– (gray) β cells. (D) Average rates of exocytosis (ØC/Øt) ± SEM evoked by Ca²⁺ dialysis in 10 WT (black bars) and 10 CaV2.3^–/– (gray bars) β cells. (E and F) Exocytosis and average ØC were recorded as in A and B, but results are from α cells, and averages represent 5 WT (black) and 3 CaV2.3^–/– (gray) α cells. (G and H) ΔC and average rates of exocytosis were recorded as in C and D, but the data are from α cells, and mean responses are from 6 WT (black) and 3 CaV2.3^–/– (gray) α cells. pF, picofarads.