JCI - Neuronal calcium sensor-1 enhancement of InsP3 receptor activity is inhibited by therapeutic levels of lithium

Neuronal calcium sensor-1 enhancement of InsP₃ receptor activity is inhibited by therapeutic levels of lithium

Christina Schlecker, Wolfgang Boehmerle, Andreas Jeromin, Brenda DeGray, Anurag Varshney, Yogendra Sharma, Klara Szigeti-Buck, Barbara E. Ehrlich

Published in Volume 116, Issue 6

J Clin Invest. 2006; 116(6):1668–1674 doi:10.1172/JCI22466

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Figure 5

PC12 cells overexpressing NCS-1 show increased intracellular calcium release after stimulation by extracellular ATP.

(A) Wild-type PC12 cells contain both NCS-1 (green) and InsP₃R1 (red). Scale bar: 50 μm. (B) Representative traces showing calcium release evoked by the addition of 50 μM ATP for 1 minute followed by a washout (dark trace: PC12 cells overexpressing NCS-1; light trace: PC12 cells transfected with empty vector). (C) Representative traces comparing the intracellular calcium release after preincubation with lithium (30 minutes, 10 mM lithium; light trace) with NCS-1 cells that were not treated with lithium (dark trace). Note that lithium did not alter the fluorescence signal but did decrease the NCS-1–dependent increase in calcium release to control levels. (D) Averaged values from at least 3 experiments.