JCI - Neuronal calcium sensor-1 enhancement of InsP3 receptor activity is inhibited by therapeutic levels of lithium

Neuronal calcium sensor-1 enhancement of InsP₃ receptor activity is inhibited by therapeutic levels of lithium

Christina Schlecker, Wolfgang Boehmerle, Andreas Jeromin, Brenda DeGray, Anurag Varshney, Yogendra Sharma, Klara Szigeti-Buck, Barbara E. Ehrlich

Published in Volume 116, Issue 6

J Clin Invest. 2006; 116(6):1668–1674 doi:10.1172/JCI22466

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Figure 4

Calcium dependence of the effect of NCS-1 and its E120Q mutant on InsP₃ R1.

Single-channel recordings of InsP₃R1 at the calcium concentrations indicated in the figure in the presence of E120Q and InsP₃ (A, left traces) or NCS-1 and 2 μM InsP₃ (A, right traces). Averaged values from at least 3 experiments for the mean open time (B) and the P_o (C) are shown for the effect of the addition of either NCS-1 (open circles) or the mutant form E120Q (filled circles). There is a calcium-independent activation as shown at low calcium concentrations after addition of E120Q and a calcium-dependent activation shown after addition of NCS-1. The purified InsP₃R1 used in this study displays no inhibition at calcium levels over the negative log of the free calcium concentration (pCa) 6.5 (black line; data taken from ref. 40).