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Neuronal calcium sensor-1 enhancement of InsP3 receptor activity is inhibited by therapeutic levels of lithium
Christina Schlecker, Wolfgang Boehmerle, Andreas Jeromin, Brenda DeGray, Anurag Varshney, Yogendra Sharma, Klara Szigeti-Buck, Barbara E. Ehrlich
Published in Volume 116, Issue 6
J Clin Invest. 2006; 116(6):1668–1674 doi:10.1172/JCI22466
This article Copyright © 2006, The American Society for Clinical Investigation
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Figure 3
Effect of NCS-1 and the E120Q mutant of NCS-1 on the activity of InsP3 R1.

(A) Single-channel recordings of InsP3R1 where downward deflections are channel openings. The top pair of traces on the left were recorded after addition of 300 nM free calcium and 2 μM InsP3, and the bottom left pair of traces show activity after addition of 2 μg/ml NCS-1 in the presence of 2 μM InsP3. In each case, the uppermost trace represents 10 seconds of continuous recording, and the bottom trace shows a 10-fold expansion of the time scale. On the right, the top pair of traces were recorded in the presence of 2 μg/ml NCS-1 in the absence of InsP3, and the bottom pair of traces were recorded after addition of 2 μg/ml E120Q, a mutant of NCS-1, in the presence of 2 μM InsP3. Averaged values from at least 3 experiments for mean open time (B) and for Po (C) of InsP3R1 corresponding to the traces shown in A. Note that NCS-1 alone did not activate InsP3R1. (D) Addition of NCS-1 (2 μg/ml) had no effect on the activity of the RyR2 activated by 300 μM calcium.

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