Neuronal calcium sensor-1 enhancement of InsP₃ receptor activity is inhibited by therapeutic levels of lithium
J. Clin. Invest. 116:6 doi:10.1172/JCI22466 [Go to this article.]

Figure 2
Coimmunoprecipitation of NCS-1 and InsP₃ R1 in rat cerebellum.

Lanes show mouse cerebellar lysate (lane 1); beads treated with cerebellar lysate but without antibody (lane 2); immunoprecipitate (IP) with anti–NCS-1 with calcium buffered (lane 3); immunoprecipitate with anti–NCS-1 with 50 μM free calcium present (lane 4); immunoprecipitate with anti-InsP₃R with calcium buffered (lane 5); and immunoprecipitate with anti-InsP₃R with 50 μM free calcium present (lane 6). Top and bottom panels show immunoblots for InsP3R1 and NCS-1, respectively.