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MyD88-dependent induction of allergic Th2 responses to intranasal antigen
Damani A. Piggott, Stephanie C. Eisenbarth, Lan Xu, Stephanie L. Constant, James W. Huleatt, Christina A. Herrick, Kim Bottomly
Published in Volume 115, Issue 2
J Clin Invest. 2005; 115(2):459–467 doi:10.1172/JCI22462
This article Copyright © 2005, The American Society for Clinical Investigation
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Figure 1

Th2 responses to intranasal protein antigen are MyD88 dependent. (A) BAL inflammatory cells of WT, MyD88-deficient, or TLR4d mice sensitized intranasally with OVA/LPS and WT mice sensitized with PBS. Exposed mice were then challenged 2 weeks later with inhaled OVA. Total bar height represents total BAL cell number, and error bars are based on total cell numbers. Stacked bars represent cell differential counts. n = 4. *P < 0.05, total BAL cell number from WT vs. MyD88-deficient and WT vs. TLR4d. One representative experiment of 4 is shown. (B) OVA-specific serum antibody responses by ELISA after challenge with OVA in WT, MyD88-deficient, and TLR4d mice sensitized as described in A. P < 0.05, WT vs. MyD88-deficient and WT vs. TLR4d, for IgE and IgG1 responses. (C) Cytokine production from lung draining lymph nodes after challenge with OVA in WT, MyD88-deficient, and TLR4d mice sensitized as described in A.

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