The MODY1 gene HNF-4α regulates selected genes involved in insulin secretion
J. Clin. Invest. Rana K. Gupta, et al. 115:1006 doi:10.1172/JCI22365 [Go to this article.]

Figure 5
Gene expression analysis in isolated islets of HNF-4α^loxP/loxP; Ins.Cre mice. (A) Levels of mRNA of MODY genes, as determined by real-time PCR. (B) Levels of mRNA of pancreatic enriched transcription factors, as determined by real-time PCR. (C) Levels of mRNA of genes involved in glucose and lipid metabolism, as determined by real-time PCR. (D) Levels of mRNA of genes involved in stimulus-secretion coupling, as determined by real-time PCR. *P < 0.05; n = 3–5 for all PCR experiments. HPRT, hypoxanthine guanine phosphoribosyl transferase. (E) Western blot analysis of HNF-1α in isolated islets normalized to α-tubulin protein levels. C, control; M, mutant. (F) Western blot analysis of Kir6.2 in isolated islets normalized to α-tubulin protein levels. For all protein quantification, n = 3, controls, and n = 2, mutants.