Clonally expanded novel multipotent stem cells from human bone marrow regenerate myocardium after myocardial infarction
J. Clin. Invest. Young-sup Yoon, et al. 115:326 doi:10.1172/JCI22326 [Go to this article.]

Figure 1
Characteristics of hBMSCs. (A) Phase-contrast and fluorescent images show a single cell per well. Original magnification, ×400. (B–D) Cells were analyzed with a FACStar flow cytometer. (B) Morphologically, most hBMSCs demonstrate round morphology with a cell size of less than 15 μm in diameter. Scale bar: 100 μm. (C) Clonally isolated hBMSCs cultured for 120 PDs were labeled with PE- or FITC-conjugated Abs against human CD29, CD44, CD73, CD105, CD90, or CD117, or Ig isotype controls. Blue lines, control Ig; red lines, specific Ab. Clonally isolated hBMSCs showed only minimal expression or no expression (<3%) of CD90, CD105, and CD117 (C). In contrast, the purchased MSCs expressed significantly higher levels of CD29, CD44, CD73, CD105, CD90, and CD117. (D) Mean TRF length of hBMSCs cultured for 5 PDs (lane 2) and 120 PDs (lane 3). No difference in mean TRF is shown. Human umbilical vein ECs (lane 4) and immortalized cell lines with high telomere length, provided by the Roche Diagnostics Corp. (lane 5), were used as controls. Lanes 1 and 6, molecular weight standards. (E) DNA ploidy analysis. hBMSCs were stained with propidium iodide and subjected to FACS analysis. Representative examples of hBMSCs cultured for 20 (left panel) and 140 (right panel) PDs demonstrate no evidence of overdiploid DNA content. Similar experiments were performed at least 3 times (A–E).