Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell–driven B cell responses
J. Clin. Invest. Hyung W. Lim, et al. 114:1640 doi:10.1172/JCI22325 [Go to this article.]

Figure 6
CD4⁺CD25⁺CD69^– Tregs are found in the IFAs and GCs of tonsils. To measure distribution of CD4⁺CD25⁺CD69^– Tregs, frozen tonsil sections were stained for CD4, CD25, and CD69 (A and B). Secondary follicles with GCs were identified by staining of serial sections with antibodies for CD57, IgD, and CD4 (not shown). Stained sections were analyzed with a confocal microscopy system (Bio-Rad Laboratories Inc. MRC-1024UV microscope, and Nikon Inc. Diaphot 300 microscope). A representative set of data from 5 independent experiments using 3 different tonsil specimens is shown. Arrows indicate CD4⁺CD25⁺CD69^– Tregs, and the circled cells are CD4⁺CD25⁺CD69⁺ T cells.