Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell–driven B cell responses
J. Clin. Invest. Hyung W. Lim, et al. 114:1640 doi:10.1172/JCI22325 [Go to this article.]

Figure 5
CD4⁺CD25⁺CD69^– Tregs switch their expression pattern of chemokine receptors and chemotactic responsiveness upon T cell activation. Tonsil mononuclear cells, freshly isolated or briefly activated for 10_14 hours with phytohemagglutinin (PHA) or for 5_10 hours with anti-CD3 and anti-CD28, were examined for their expression of chemokine receptors (A, B, and D) or for chemotactic responsiveness (C and E). In D, the chemokine receptors were examined by 1- or 2-step staining methods to avoid cross-reaction with the anti-CD3 and anti-CD28 antibodies used to activate the cells; these methods are less sensitive than the 3-step method used for A and B. The data obtained from 3 independent experiments were combined, and averages (and SEM in A) are shown. The background percent migration rates (averages and SEM, 3 experiments) for the 4 cell subsets were 23 ± 4.4 (CD4⁺CD25^–CD69⁺), 16.3 ± 3.2 (CD4⁺CD25⁺CD69⁺), 23 ± 1.0 (CD4⁺CD25⁺CD69^– Treg), and 11 ± 3.2 (CD4⁺CD25^–CD69^–). *Significant differences between the 2 samples. The P values were 0.024 (a), 0.042 (b), 0.032 (c), 0.019 (d), 0.045 (e), 0.033 (f), 0.041 (g), 0.002 (h), 0.049 (i), 0.021 (j), 0.023 (k), 0.009 (l), 0.004 (m), and 0.006 (n).