Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell–driven B cell responses
J. Clin. Invest. Hyung W. Lim, et al. 114:1640 doi:10.1172/JCI22325 [Go to this article.]

Figure 2
Phenotype of CD4⁺CD25⁺CD69^– Tregs. (A) Freshly isolated tonsil T cell subsets were stained with antibodies to TGF-β1, CTLA-4, CD62 ligand (CD62L), and CD45RA. Stained cells were analyzed by a FACSCalibur. The histograms show representative data, and the graphs show combined data (averages and SEM, n = 3). Mean fluorescent intensity levels for the antigens expressed by the 2 populations are also shown in parentheses. (B) Expression of Foxp3 and GITR by CD4⁺CD25^–CD69⁺ cells, CD4⁺CD25⁺CD69⁺ cells, CD4⁺CD25⁺CD69^– Tregs, and CD4⁺CD25^–CD69^– T cells, determined by RT-PCR. (C) Foxp3 expression by T cell subsets measured by quantitative real-time RT-PCR. Foxp3 expression was normalized for β-actin levels. Representative data (averages and SD of triplicate measurements) from 4 independent experiments are shown.