Identification of cellular deoxyhypusine synthase as a novel target for antiretroviral therapy
J. Clin. Invest. Ilona Hauber, et al. 115:76 doi:10.1172/JCI21949 [Go to this article.]

Figure 7
Inhibition of HAART-resistant HIV-1 isolates. PM1 cells were incubated for 6 days in the presence of 1.0 μM CNI-1493 or DMSO and then infected with various types of antiretroviral drug–resistant viruses. Results of the phenotypic drug sensitivity assay (red areas in the virograms; for details see text) are shown. The resistance-conferring mutations in the various viral genomes (genotypes), as determined by DNA sequencing, are summarized in the supplemental data section. (A) Infected cells were incubated in CNI-1493 at concentrations of 1.0 μM and 0.5 μM, or DMSO (control). Inhibition of virus replication (in percent) and drug toxicity (as measured by MTT assay) at days 6 and 12 are indicated in the right panels. (B) Three-week time course of inhibition of an “omni-resistant” virus isolate (FE9) by CNI-1493. p24 antigen level (p24Ag/CNI-1493 and p24Ag/DMSO control) and cell viability (MTT/CNI-1493 and MTT/DMSO control) were determined at days 6, 9, 12, 16, and 20 after infection (graphs). PI, protease inhibitor; NRTI, nucleoside reverse transcriptase inhibitor; NNRTI, non-nucleoside reverse transcriptase inhibitor; AZT, zidovudine; DDC, zalcitabine; ddI, didanosine; D4T, stavudine; 3TC, lamivudine.