Multiple environmental and genetic factors influence skeletal muscle PGC-1α and PGC-1β gene expression in twins
J. Clin. Invest. Charlotte Ling, et al. 114:1518 doi:10.1172/JCI21889 [Go to this article.]

Figure 1
Effects of insulin and age on human skeletal muscle PGC-1α and PGC-1β mRNA levels. Skeletal muscle specimens were taken from young (n = 86) and elderly (n = 68) twins before and after a hyperinsulinemic clamp. RNA was prepared and analyzed for PGC-1α (A) and PGC-1β (B) mRNA expression, together with the internal standard cyclophilin A using TaqMan Real-Time PCR with an ABI 7900 system. The PGC-1α/cyclophilin A ratio and the PGC-1β/cyclophilin A ratio were calculated for each sample, and the ratios are presented in the figure. Results are expressed as the mean ± SEM. *P ≤ 0.05.