High-level β-globin expression and preferred intragenic integration after lentiviral transduction of human cord blood stem cells
J. Clin. Invest. Suzan Imren, et al. 114:953 doi:10.1172/JCI21838 [Go to this article.]

Figure 1
Schematic diagram of the self-inactivating β^A-T87Q-globin lentiviral vector used in this study. Features include the HIV LTR; the packaging signal (ψ⁺); the central polypurine tract (cPPT); the rev-responsive element (RRE); the β-globin promoter, from SnaBI to the cap site (βp); the 3′ β-globin enhancer (to the downstream AvrII site); the 372-bp IVS2 deletion (indicated by the triangle); the β^A-T87Q mutation (ACA Thr [β⁸⁷ Thr] to CAG Gln [γ⁸⁷ Gln]); and the DNase I_hypersensitive sites, SmaI to XbaI (HS2), SacI to PvuII (HS3), and StuI to SpeI (HS4) of the β-globin LCR; 2 stop codons in the ψ⁺ packaging signal; the 400-bp deletion in U3 of the right-hand HIV LTR; and the rabbit β-globin polyA signal. The locations of the β^A-T87Q-globin transgene-specific primers in exons 2 and 3 (E2, E3) are indicated by arrowheads.