Atypical PKC-ζ regulates SDF-1–mediated migration and development of human CD34⁺ progenitor cells
J. Clin. Invest. Isabelle Petit, et al. 115:168 doi:10.1172/JCI21773 [Go to this article.]

Figure 4
SDF-1 induces PKC-ζ activation. (A) G2 cells were stimulated with SDF-1, and a phosphorylated form of PKC-ζ (pPKC-ζ) was detected by immunoblot assay. (B–D) CD34⁺ cells, untreated (B) or stimulated with SDF-1 (200 ng/ml) either pretreated with wortmannin (D) or not (C), were stained by indirect immunolabeling with anti–PKC-ζ antibody. Arrows indicate membranal PKC-ζ accumulation. Scale bar: 5 μm. (E) SDF-1–induced PKC-ζ kinase activity was assayed in vitro in the presence of myelin basic protein (MBP) and ³²P-ATP. (F) Cells, either untreated or pretreated with wortmannin (W; 50 nM), U7322 (U; 10 μM), or D609 (10 μM), were subjected to SDF-1–induced transwell migration assay. Migration of control cells to medium only (–) and to SDF-1 is indicated. Data shown in D are average ± SD of 3 experiments; *P < 0.05.