Atypical PKC-ζ regulates SDF-1–mediated migration and development of human CD34+ progenitor cells
J. Clin. Invest. Isabelle Petit, et al. 115:168 doi:10.1172/JCI21773 [
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Figure 3Impaired response of pre-B ALL B1 cells to SDF-1. (
A) CXCR4 expression on G2 and B1 cells was assayed by flow cytometry. (
B) SDF-1–induced calcium mobilization. Cells were loaded with Fluo-3 and stimulated twice with SDF-1 (0.5 μg/ml) and analyzed by flow cytometry. (
C) Transwell migration toward SDF-1 (125 ng/ml). (
D and
E) Spreading and motility under laminar shear flow of G2 (
D) and B1 cells (
E) on VCAM-1/SDF-1–coated surfaces. Original magnification, ×10. Black arrows indicate spread and thus dark cells; white arrows indicate non-spread rounded cells that appear bright in phase light. (
F) Actin polymerization following SDF-1 (300 ng/ml) stimulation in G2 and B1 cells was measured by phalloidin-FITC staining. (
G) Detection of PKC-ζ mRNA expression by RT-PCR in neutrophils, B1 cells, and G2 cells.
