Atypical PKC-ζ regulates SDF-1–mediated migration and development of human CD34⁺ progenitor cells
J. Clin. Invest. Isabelle Petit, et al. 115:168 doi:10.1172/JCI21773 [Go to this article.]

Figure 1
Effect of PKC inhibitors on SDF-1–induced migration. CD34⁺ cells (A and C) or G2 cells (B and D) were preincubated for 30 minutes with PKC inhibitors staurosporine (ST), GF 109203X (GF), or indicated concentrations of chelerythrine chloride (CC) (A and B) or for 1 hour with 10 μM of either PS-α/β, PS-ε, or indicated concentrations of PS-ζ peptides (C and D). SDF-1–induced migration was determined by the transwell assay. Migration of control cells to medium only (–) and to SDF-1 is indicated. Data show average ± SD of at least 3 experiments; *P < 0.05. (E) G2 and U937 cells transfected with GFP–PKC-ζ–expressing plasmid were subjected to transwell migration to SDF-1. Where indicated (white bars), transfected cells were preincubated for 1 hour with PS-ζ peptides (10 μM). Fold increase migration represents the ratio of the number of GFP–PKC-ζ migrating cells to the number of migrating mock-transfected cells. Insert indicates the percentage of GFP⁺ G2 cells in the migrating (Migr.) and nonmigrating (Nonmigr.) cell populations. Results are average ± SD of 2 independent experiments for each cell line; *P < 0.05.