Dynamic changes in Mcl-1 expression regulate macrophage viability or commitment to apoptosis during bacterial clearance
J. Clin. Invest. Helen M. Marriott, et al. 115:359 doi:10.1172/JCI21766 [
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Figure 4Mcl-1
Exon-1 expression is an upstream event in pneumococcal-associated but not UV-associated macrophage apoptosis. (
A) Western blot of total protein from MDMs 20 hours after infection in the presence (+) or absence (–) of zVADfmk (zVAD) or
N-benzyloxycarbonyl–Phe-Ala fluoromethyl ketone (zFA), probed with anti–Mcl-1, anti–Bcl-xL, and anti-GAPDH antibodies. Data is representative of 4 donors. (
B) Western blot of membrane and nuclear fractions from MDMs 20 hours after infection, probed with anti–Mcl-1 antibody. Data represent 2 donors and 3 independent experiments. (
C) Nuclear morphology, detected by DAPI staining, in control MDMs (left panel) or MDMs 12 hours after UV irradiation (right panel). Levels of apoptosis were 1% in control MDMs and 34% in UV-irradiated MDMs. (
D) Western blot of total protein from MDMs at the indicated time points after UV irradiation, probed with anti–Mcl-1, anti-p53, and anti-actin antibodies. The asterisks mark the expected positions of Mcl-1
Exon-1. Data are representative of 3 donors.
