Dynamic changes in Mcl-1 expression regulate macrophage viability or commitment to apoptosis during bacterial clearance
J. Clin. Invest. Helen M. Marriott, et al. 115:359 doi:10.1172/JCI21766 [Go to this article.]

Figure 2
Mitochondrial features of apoptosis are observed in macrophages infected with pneumococci. (A) Western blot of the cytosolic and mitochondrial (Mit) fractions from MDMs at the indicated time points after infection with pneumococci or mock infection probed with anti–cytochrome c and anti-actin antibodies. Results are derived from 2 donors and are representative of 2 independent experiments. (B) Western blot of the cytosolic fractions from MDMs 20 hours after infection in the presence (+) or absence (–) of zVADfmk or N-benzyloxycarbonyl–Phe-Ala fluoromethyl ketone (zFA) probed with anti–cytochrome c and anti-actin antibodies. Results are derived from 2 donors and are representative of 2 independent experiments. (C) MDMs were stained with JC-1 and Hoechst 33342 (Ho33342) 20 hours after infection. Loss of °ψ_m is represented as a loss of punctate red staining, and nuclear morphology is detected by Hoechst 33342 staining. Data are representative of 3 donors. (D) Histograms representing °ψ_m of MDMs at the indicated time points after infection, stained with JC-1. The percentage of cells with preservation of °ψ_m (indicated by the M1 marker) is shown. Results are representative of 3 independent experiments. (E) Western blot of nuclear fractions from MDMs 20 hours after infection, probed with anti-AIF and Histone H1 antibodies. Data are representative of 3 independent experiments.