Dynamic changes in Mcl-1 expression regulate macrophage viability or commitment to apoptosis during bacterial clearance
J. Clin. Invest. Helen M. Marriott, et al. 115:359 doi:10.1172/JCI21766 [
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Figure 1Mcl-1 protein levels demonstrate biphasic variation in association with pneumococcal infection of macrophages. (
A) Total RNA was extracted from MDMs 20 hours after mock infection (Spn–) or infection with pneumococci (Spn+), and Mcl-1 mRNA was detected by RNase protection assay. The results presented are typical of 5 donors tested, but the duration of mRNA elevation varied among donors. L32 and GAPDH served as loading controls. (
B) Western blot of total protein from MDMs at the indicated time points after infection, probed with anti–Mcl-1 and anti-actin antibodies. Data are representative of 7 independent experiments. (
C) Mcl-1 band density from a series of MDMs as in
B, quantified by densitometry (
n = 7).
P < 0.05, Mcl-1 at 6 hours vs. 12 hours;
P < 0.001, 6 hours vs. 20 hours; Student’s
t test. (
D) Levels of nuclear apoptosis in the same series of MDMs as in
C.
P < 0.001, apoptosis at 6 hours vs. 16 hours, Student’s
t test.
