JCI - Reversing the defective induction of IL-10–secreting regulatory T cells in glucocorticoid-resistant asthma patients

Reversing the defective induction of IL-10–secreting regulatory T cells in glucocorticoid-resistant asthma patients

Emmanuel Xystrakis, Siddharth Kusumakar, Sandra Boswell, Emma Peek, Zoë Urry, David F. Richards, Tonye Adikibi, Carol Pridgeon, Margaret Dallman, Tuck-Kay Loke, Douglas S. Robinson, Franck J. Barrat, Anne O’Garra, Paul Lavender, Tak H. Lee, Christopher Corrigan, Catherine M. Hawrylowicz

Published in Volume 116, Issue 1

J Clin Invest. 2006; 116(1):146–155 doi:10.1172/JCI21759

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Figure 1

Polyclonal activation of human CD4⁺ T cells in the presence of dexamethasone plus vitamin D3 induces IL-10 expression, which does not correlate with expression of the transcription factor Foxp3.

(A) CD4⁺ T cells from an SS asthma patient were cultured with APCs, anti-CD3, IL-2, and IL-4 alone (medium) or together with 10^–7M Vitamin D3 and 10^–7M dexamethasone (vit/dex). Cells were restimulated at 7 days with PMA and ionomycin for 4 hours, and coexpression of IL-10 with IL-2, IL-4, IFN-γ, or GM-CSF was analyzed by flow cytometry. Data are representative of 5 experiments. (B) Real-time RT-PCR was performed on PBMCs, freshly isolated CD4⁺CD25^– and CD4⁺CD25⁺ T cells, and IL-10–secreting Tregs harvested after 14 days in culture to compare expression of Foxp3 and IL-10. Data are representative of 3 experiments. HPRT, hypoxanthine-guanine phosphoribosyl transferase.