Mitochondrial remodeling in adipose tissue associated with obesity and treatment with rosiglitazone
J. Clin. Invest. Leanne Wilson-Fritch, et al. 114:1281 doi:10.1172/JCI21752 [Go to this article.]

Figure 7
Western blotting of mitochondrial proteins. Isolated adipocytes were lysed in sample buffer containing 1% SDS. Equal concentrations of total protein (20 μg) were analyzed by SDS-PAGE and Western blotting using antibodies against mitochondrial (CytC, cytochrome c; COX, cytochrome oxidase subunit II; mHsp60) and non-mitochondrial (Chc, clathrin heavy chain; Actin) proteins. Except where indicated by the asterisk, all samples were from ob/ob mice with the ages and conditions indicated. Band intensities were quantified using Photoshop 7 software as described in Methods and were plotted. Similar results were obtained in three experiments.