Mitochondrial remodeling in adipose tissue associated with obesity and treatment with rosiglitazone
J. Clin. Invest. Leanne Wilson-Fritch, et al. 114:1281 doi:10.1172/JCI21752 [
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Figure 4Live cell imaging of mitochondria with MitoTracker Green FM. Adipocytes were isolated from epididymal fat pads of 26-week-old C57BL/6J or
ob/ob mice that were either left untreated or were treated with rosiglitazone for 14 days. Cells were incubated with 100 nM MitoTracker Green FM for 30 minutes and 25 μl of the cell suspension was placed on a microscope slide. A glass coverslip was floated on the cell suspension and images were acquired within 5 minutes using a conventional wide-field microscope fitted with a ×40 or ×100 Nikon plan-apo objective.
