Src promotes estrogen-dependent estrogen receptor α proteolysis in human breast cancer
J. Clin. Invest. Isabel Chu, et al. 117:2205 doi:10.1172/JCI21739 [
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Figure 3Src promotes estrogen-dependent ERα transcriptional activity. (
A–
C) MCF-7 was transfected with ERE luciferase reporter and either PCI-Src Y530F or empty vector control and then stimulated with estradiol. Shown are (
A) ERE luciferase activity as well as ERα and Src levels (
B) before and (
C) 4 hours after Src transfection, estradiol addition, or both. (
C) Relative ERE luciferase activity corrected for ERα level. (
D and
E) MCFpINDSrc2 cells were estrogen depleted for 72 hours, and cSrc was induced or not for 24 hours prior to the addition of estradiol (Est) or estradiol plus tamoxifen (Est + Tam). (
D and
E) QPCR of cellular (
D) GREB1 and (
E) pS2.
