Hypothalamic PI3K and MAPK differentially mediate regional sympathetic activation to insulin
J. Clin. Invest. Kamal Rahmouni, et al. 114:652 doi:10.1172/JCI21737 [Go to this article.]

Figure 2
Insulin activates PI3K and MAPK in the hypothalamus. Rats were killed at the indicated time points after ICV administration of 500 mU of insulin or vehicle, and total proteins were extracted from the mediobasal hypothalamus and assayed. (A) Effect of ICV administration of insulin on the interaction of IRS-1 and the p85 subunit of PI3K in the mediobasal hypothalamus. Top, Western blot of p85 PI3K immunoprecipitated with IRS-1. Bottom, densitometric analysis of the immunoreactive bands for p85 PI3K immunoprecipitated with IRS-1, expressed as change relative to that of vehicle-treated group. (B) Effect of ICV administration of insulin on the total MAPK (p42 and p44 MAPK) and phosphorylated forms of MAPK (P-p42 and P-p44 MAPK) in the mediobasal hypothalamus. Top, Western blot of the total and phosphorylated forms of MAPK. Bottom, densitometric analysis of the immunoreactive bands for phosphorylated form of MAPK, expressed as change relative to that of vehicle-treated group. Data represent means ± SEM of 4 rats per group. *P < 0.001 versus the vehicle-treated group. AU, arbitrary units.