EVI1 induces myelodysplastic syndrome in mice
J. Clin. Invest. Silvia Buonamici, et al. 114:713 doi:10.1172/JCI21716 [Go to this article.]

Figure 2
EVI1 induces BM hypercellularity, dyserythropoiesis, erythroid and megakaryocytic hyperplasia, and apoptosis. Sections of normal spleen tissue (A and C) show normal white and red pulp. In comparison, EVI1-positive spleen tissue shows an expansion of red pulp and erythroid hyperplasia (B and dark cells in D). (E) Prussian blue iron staining of normal spleen tissue does not identify extensive iron deposition. (F) Iron depositions are evident in a section of EVI1-positive spleen tissue stained with Prussian blue. (H and J) Sections of EVI1-positive spleen tissue stained with cleaved caspase-3 antibody demonstrate the presence of apoptosis. (G and I) Normal spleen stained with cleaved caspase-3 antibody. (K and L) Control BM biopsy specimen shows normal cellularity and trilineage hematopoiesis. (M) In contrast, the EVI1-positive BM appears hypercellular, with erythroid and megakaryocytic hyperplasia. The BM dyserythropoiesis in EVI1-positive BM aspirates is shown (N), where the arrows point to nuclear irregularity and nuclear budding of erythroid precursors. (O) A PB smear of a control mouse is shown. In contrast, the PB smear of an EVI1-positive mouse shows anisopoikilocytosis (P), increased number of polychromatophilic rbc’s (Q), and Howell-Jolly bodies (R). Magnification, ×10 (A, B, E, F, G, H, K, M); ×40 (C, D, I, J, L, N, O, P, Q); ×100 (R).