Increased oxidative stress in obesity and its impact on metabolic syndrome
J. Clin. Invest. Shigetada Furukawa, et al. 114:1752 doi:10.1172/JCI21625 [Go to this article.]

Figure 4
Decreased mRNA expressions and activities of antioxidant enzymes in WAT of obese KKAy mice. (A) The mRNA expressions of Cu,Zn-SOD, Gpx, and catalase in WAT, liver, and skeletal muscle of C57BL/6 (white bars) and KKAy (black and gray bars) mice at 7 and 13 weeks of age. Values are normalized to the level of cyclophilin mRNA, and expressed as mean ± SEM (n = 6–8). (B) Immunoblot analysis of the amount of Cu,Zn-SOD protein in WAT, liver, and skeletal muscle of C57BL/6 and KKAy mice at 7 and 13 weeks of age. (C) Total SOD activities (left) and GPx activities (right) in WAT, liver, and skeletal muscle of C57BL/6 (white bars) and KKAy (black and gray bars) mice at 7 and 13 weeks of age. Values are expressed as mean ± SEM (n = 6–8). (D) Model illustrating increased production of oxidative stress in accumulated fat. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with C57BL/6 mice.