Increased oxidative stress in obesity and its impact on metabolic syndrome
J. Clin. Invest. Shigetada Furukawa, et al. 114:1752 doi:10.1172/JCI21625 [
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Figure 3Dysregulated expressions of adipose genes and increased expressions of NADPH oxidase subunits in WAT of obese KKAy mice. (
A) The mRNA expressions of adiponectin, TNF-α, PAI-1, and PPARγ in WAT of C57BL/6 (white bars) and KKAy (black bars) mice. The mRNA amounts were quantified by real-time PCR. Values are normalized to the level of cyclophilin mRNA, and expressed as mean ± SEM (
n = 6–8). (
B) The mRNA expressions of NADPH oxidase subunits in various mouse tissues. The mRNA amounts were quantified by real-time PCR, using total RNA extracted from 8 tissues of C57BL/6 mice at 12 weeks of age. Values are normalized to the level of 18S ribosomal RNA. (
C and
D) The mRNA expressions of NADPH oxidase subunits and PU.1 in WAT (
C), liver (
D, left) and skeletal muscle (
D, right) of C57BL/6 (white bars) and KKAy (black bars) mice at 7 and 13 weeks of age. Values are normalized to the level of cyclophilin mRNA, and expressed as mean ± SEM (
n = 6–8). **
P < 0.01 and ***
P < 0.001 compared with C57BL/6 mice. BAT, brown adipose tissue.
