JCI - c-Cbl–deficient mice have reduced adiposity, higher energy expenditure, and improved peripheral insulin action

c-Cbl–deficient mice have reduced adiposity, higher energy expenditure, and improved peripheral insulin action

Juan C. Molero, Thomas E. Jensen, Phil C. Withers, Michelle Couzens, Herbert Herzog, Christine B.F. Thien, Wallace Y. Langdon, Ken Walder, Maria A. Murphy, David D.L. Bowtell, David E. James, Gregory J. Cooney

Published in Volume 114, Issue 9

J Clin Invest. 2004; 114(9):1326–1333 doi:10.1172/JCI21480

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Figure 6

Representative electron micrographs of soleus muscle from WT and c-Cbl–deficient mice (A), showing the subsarcolemmal mitochondria population. Scale bar: 1 μm. The histogram shows the average mitochondrial size from more than 100 mitochondria from matching sections of muscles from WT (black bars) and c-Cbl–deficient (white bars) mice. (B and C) Muscle homogenates were immunoblotted with either antibodies specific for UCP3, pSer79-ACC, ACC, and PGC1α (5 and 15 μg total protein) (B) or antibodies specific for AMPK and pThr172-AMPK (60 μg total protein) (C). The fold increase in protein levels observed in c-Cbl^–/– mice compared with WT animals, as well as the result of the statistical analysis of the data, is shown at the right of each lane. Data represent the mean ± SEM of 6 (C) or 12 (B) animals in each group. (D) Histograms show ACC phosphorylation as a ratio of total ACC (top) and AMPK phosphorylation as a ratio of total AMPK (bottom) in quadriceps from WT and c-Cbl^–/– mice. *P < 0.05, **P < 0.01.