Effects of TP or IP deficiency on ICAM-1 and PECAM-1 expression in the ECs overlying atheromatous lesions of apoE-deficient mice. (A) Representative immunostaining for ICAM-1 and PECAM-1 in cross-sections from apoE^_/_ (left panels), apoE^_/_TP^_/_ (middle panels), and apoE^_/_IP^_/_ (right panels) mice. Mice were sacrificed at 20 weeks of age. Cross-sections were stained with specific antibodies against ICAM-1 (red; lower panels), PECAM-1 (red; upper panels), and smooth muscle α-actin (green). Scale bars: 20 μm. (B) Representative immunostaining of aortic arch lesions and neighboring intact areas for ICAM-1 and PECAM-1 in en face preparations of apoE^_/_ (left panels), apoE^_/_TP^_/_ (middle panels) and apoE^_/_IP^_/_ (right panels) mice. En face preparations were stained with specific antibodies against ICAM-1 (middle panels) and PECAM-1 (upper panels). Mice were sacrificed at 20 weeks of age. Scale bars: 10 μm. Merge, merged images. (C and D) Quantification of ICAM-1 (C) and PECAM-1 (D) expression in ECs overlying aortic arch lesions and neighboring intact areas of apoE^_/_, apoE^_/_TP^_/_, and apoE^_/_IP^_/_ mice. Data are means ± SEM (n = 8 each). *P < 0.05 and **P < 0.01 for bracketed comparisons.