Induction of dermal-epidermal separation in mice by passive transfer of antibodies specific to type VII collagen
J. Clin. Invest. Cassian Sitaru, et al. 115:870 doi:10.1172/JCI21386 [
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Figure 8Dermal-epidermal separation is dependent on the Fc portion of antibodies specific to type VII collagen. (
A) Subepidermal blister in a skin biopsy from a mouse receiving, over a period of 12 days, 6 injections of IgG, each containing 7.5 mg of intact IgG from immune rabbit serum. (
B) In contrast, no histological changes in a mouse injected with F(ab′)
2 fragments generated by pepsin digestion of pathogenic rabbit IgG (H&E, magnification, ×200). IF analysis of perilesional skin from a diseased mouse revealed positive staining using both anti-Fab (
C) and anti-Fc (
E) FITC-conjugated antibodies. In a mouse injected with F(ab′)
2 fragments, staining was observed with the Fab- (
D) but not with the Fc-specific (
F) conjugate (×400).
