Induction of dermal-epidermal separation in mice by passive transfer of antibodies specific to type VII collagen
J. Clin. Invest. Cassian Sitaru, et al. 115:870 doi:10.1172/JCI21386 [
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Figure 4Rabbit IgG specific to type VII collagen binds to the basement membrane and fixes complement in vivo. IF microscopy, performed on frozen sections of a perilesional mouse skin biopsy (
A and
B) and murine esophagus (
D) reveals linear deposition of rabbit IgG (
A and
D) and murine C3 (
B) at the basement membrane in a diseased mouse. (
C) IF analysis of lesional skin from a diseased mouse shows rabbit IgG deposits on both epidermal and dermal sides of the cleavage. No deposits of rabbit IgG (
E) or murine C3 (
F) are detected at the DEJ of a mouse injected with control rabbit IgG (magnification, ×250).
