Induction of dermal-epidermal separation in mice by passive transfer of antibodies specific to type VII collagen
J. Clin. Invest. Cassian Sitaru, et al. 115:870 doi:10.1172/JCI21386 [
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Figure 3IgG to murine type VII collagen induces cutaneous lesions in adult mice. (
A) Extensive skin lesions, including blisters, erosions, and epidermal detachment, developed in a BALB/c
nu/nu mouse receiving, over a period of 10 days, 5 injections of IgG, each containing 7.5 mg of IgG, from a rabbit immunized against murine type VII collagen. Injections into the back of the mouse induced (
B) spontaneous blisters on the left ear and, upon tangential pressure, (
C) epidermal detachment on the right ear. (
D) Epidermal detachment was also elicited on tail skin, and (
E) the epidermis could subsequently be easily lifted up from the dermis. (
F) Erosions covered by crusts on the hind limb. (
G–
I) A control mouse challenged with the same dose of normal rabbit IgG showed no skin alterations.
