Induction of dermal-epidermal separation in mice by passive transfer of antibodies specific to type VII collagen
J. Clin. Invest. Cassian Sitaru, et al. 115:870 doi:10.1172/JCI21386 [
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Figure 1Schematic organization of human type VII collagen and cDNA constructs generated for this study for expressing recombinant peptides of the NC1 domain. Type VII collagen is composed of 3 identical α chains, each consisting of a central triple helical collagenous domain, flanked by a large amino-terminal noncollagenous domain (NC1) and a smaller carboxy-terminal noncollagenous domain (NC2). Two molecules form antiparallel tail-to-tail dimers stabilized by disulfide bonding through a carboxy-terminal overlap between NC2 domains. Three fragments of murine type VII collagen cDNA were cloned in pGEX-6P-1 and expressed in E. coli. Amino acid residue numbers are shown below the fragments.
