Vasohibin as an endothelium-derived negative feedback regulator of angiogenesis
J. Clin. Invest. Kazuhide Watanabe, et al. 114:898 doi:10.1172/JCI21152 [
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Figure 4KIAA1036 is preferentially expressed in ECs. (
A) Expression of KIAA1036 in cultured cells. Cells were preincubated in 0.1% FCS/α-MEM for 12 hours and then stimulated with growth factors as follows: HUVECs with VEGF (1 nM), HASMCs with PDGF (1 nM), human fibroblasts with FGF-2 (2 nM), and keratinocytes with EGF (1 nM). Thereafter, total RNA was obtained and Northern blotting for vasohibin was performed. (
B) Expression of KIAA1036 in vivo was examined by multiple-tissue Northern blot. (
C) Localization of KIAA1036 protein in the placenta. Sections of human placenta were subjected to immunostaining. Anti_human CD31 mAb, anti_KIAA1036 mAb, or mouse IgG was used as the primary Ab. Scale bars: 100 μm. (
D) Expression of KIAA1036 in human embryo. Northern blotting for vasohibin was performed using a human developmental total RNA Northern blot.
