Genetic ablation of Nrf2 enhances susceptibility to cigarette smoke–induced emphysema in mice
J. Clin. Invest. Tirumalai Rangasamy, et al. 114:1248 doi:10.1172/JCI21146 [Go to this article.]

Figure 4
Increased sensitivity of Nrf2^–/– mice to oxidative stress after CS exposure. (A) Immunohistochemical staining for 8-oxo-dG in lung sections from the mice exposed to CS (6 months) (n = 5 per group). Lung sections from the CS-exposed Nrf2^–/– mice show increased staining for 8-oxo-dG (indicated by arrows) when compared with lung sections from CS-exposed Nrf2^+/+ mice and the respective air-exposed control mice. Magnification, ×40. (B) Quantification of 8-oxo-dG–positive alveolar septal cells in lungs after 6 months of CS exposure. The number of cells that reacted with anti–8-oxo-dG antibody was significantly higher in the lung tissues of the CS-exposed Nrf2^–/– mice than in the lung tissues of the CS-exposed Nrf2^+/+ mice and air-exposed control mice. Values (positive cells/mm alveolar length) represent mean ± SEM. *P – 0.05 vs. CS-exposed Nrf2^+/+ mice. (C) Immunohistochemical staining with normal mouse-IgG1 antibody in lung sections from air- or CS-exposed Nrf2^+/+ and Nrf2^–/– mice. Magnification, ×40. Scale bars: 25 μm.