Dopaminergic neurons generated from monkey embryonic stem cells function in a Parkinson primate model
J. Clin. Invest. Yasushi Takagi, et al. 115:102 doi:10.1172/JCI21137 [Go to this article.]

Figure 3
DA neurons differentiated from ES cell–derived neurospheres. (A–D) Differentiated spheres treated with FGF2 and FGF20 were stained with antibodies against TH (red) and TuJ1 (green). Scale bars: 50 μm. (E) The proportion of TH-positive cells to TuJ1-positive cells is expressed as the mean ± SD of 3 to 5 independent cultures. *P < 0.05. (F) RT-PCR for mesencephalic DA neuron markers Pax2, Ptx3, Nurr1, Lmx1b, and TH in cells treated with FGF2 and FGF20 (left) or FGF2 and EGF (right). (G) HPLC measuring concentration of dopamine released by SDIA- and FGF2- and FGF20-treated monkey ES cells in response to high K⁺ depolarizing stimuli (blue line). Dopamine standard, red line.