Anti-C1q autoantibodies deposit in glomeruli but are only pathogenic in combination with glomerular C1q-containing immune complexes
J. Clin. Invest. Leendert A. Trouw, et al. 114:679 doi:10.1172/JCI21075 [
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Figure 2Effects of administration of anti-C1q mAb to naive mice. (
A) C1q sandwich ELISA for the detection of serum C1q levels following administration of anti-C1q mAb and control mAb.(
B) Immunofluorescence analysis of mouse kidneys stained for the presence of mouse IgG and mouse C1q following administration of JL-1 or control mAb to WT or C1q
–/– mice. Original magnification, ×400. (
C) Quantification of immunofluorescence analysis of the deposition of IgG and C1q in WT mice. Data are expressed as arbitrary units (aU), as described in Methods. (
D) Quantification of glomerular granulocyte influx. Depicted on the
y axis are granulocytes per glomerular cross section (Gr/glom) at 24 hours after injection. (
E) Assessment of albuminuria by ELISA. Alb, albumin.
