Overexpression of Insig-1 in the livers of transgenic mice inhibits SREBP processing and reduces insulin-stimulated lipogenesis
J. Clin. Invest. Luke J. Engelking, et al. 113:1168 doi:10.1172/JCI20978 [Go to this article.]

Figure 6
In vivo synthesis rates of fatty acids (A) and sterols (B) in the livers and kidneys of WT and TgInsig-1 mice subjected to fasting and refeeding. Mice (16-week old male, five per group) were either fasted for 12 hours or fasted for 12 hours and then refed a high-carbohydrate/low-fat diet for 12 hours prior to study. Each mouse was then injected intraperitoneally with ³H-labeled water (50 mCi in 0.25 ml of isotonic saline). One hour later the tissues were removed for measurement of ³H-labeled fatty acids and digitonin-precipitable sterols. Each bar represents the mean ± SEM of values from five mice. The levels of statistical significance (Student’s t test) between the WT and TgInsig-1 groups are shown as P values. F, fasted group; R, refed group.