Overexpression of Insig-1 in the livers of transgenic mice inhibits SREBP processing and reduces insulin-stimulated lipogenesis
J. Clin. Invest. Luke J. Engelking, et al. 113:1168 doi:10.1172/JCI20978 [Go to this article.]

Figure 4
Effect of fasting and refeeding on SREBP and Insig proteins in the livers of WT and TgInsig-1 mice. Sixteen-week-old male mice (five per group) were subjected to fasting and refeeding as described in Methods. The WT mice were littermates of the transgenic mice. A detailed description of these mice is provided in Supplemental Table 2. The nonfasted group (NF) was fed a chow diet ad libitum, the fasted group (F) was fasted for 12 hours, and the refed group (R) was fasted for 12 hours and refed a high-carbohydrate/low-fat diet for 12 hours prior to study. Nuclear extract fractions were prepared from pooled livers (five mice per group); membrane fractions were prepared individually and pooled as described in Methods. Aliquots of membrane and nuclear extract fractions (45 μg) were subjected to SDS-PAGE and immunoblot analysis as described in Methods and Figures 1 and 2. Asterisks denote nonspecific bands. Open triangle denotes endogenous mouse Insig-1 (28 kDa) (lanes 1–3); arrows denote transgenic human Insig-1 (doublet of 30 kDa and 26 kDa) (lanes 4–6).