Suppression of autoimmune disease after vaccination with autoreactive T cells that express Qa-1 peptide complexes
J. Clin. Invest. Vily Panoutsakopoulou, et al. 113:1218 doi:10.1172/JCI20772 [Go to this article.]

Figure 1
Qa-1^b expression on CD4 cell vaccine is essential for protection. Effects of monoclonal anti–Qa-1 Ab on TCV in vitro (A) and in vivo (B). (A) C.AL-20 mice were vaccinated with CD4 cells isolated from HSV-1–infected C.AL-20 mice. A group of mice was vaccinated with the same CD4 cells but incubated with blocking anti–Qa-1 mAb (open squares) or isotype control (filled circles) or were nonvaccinated controls (open circles) as described in Methods. Two weeks later, vaccinated mice were ocularly infected with HSV-1 (KOS) and scored for HSK. Each group consisted of ten mice, and results are representative of three independent experiments. (B) C.AL-20 mice were intraperitoneally injected with anti–Qa-1 mAb (10 μg/mouse) at the time of TCV (open squares) or isotype control (filled circles) or were nonvaccinated controls (open circles). Two weeks later, vaccinated mice were ocularly infected with HSV-1 (KOS) and scored for HSK. Each group consisted of 10–20 mice, and results are representative of three independent experiments. (C) Qa-1 sorting. C.AL-20 mice were vaccinated with CD4 cells isolated from HSV-1–infected C.AL-20 mice. These CD4 cells were sorted according to their level of Qa-1 surface expression (high, filled triangles, versus low, filled squares) or nonvaccinated controls (open circles). Both groups were ocularly infected with HSV-1 (KOS) and scored for HSK as described in Methods. Each group consisted of 10–20 mice. Data are representative of two independent experiments.