JCI - TNF-α is crucial for the development of autoimmune arthritis in IL-1 receptor antagonist

TNF-α is crucial for the development of autoimmune arthritis in IL-1 receptor antagonist–deficient mice

Reiko Horai, Akiko Nakajima, Katsuyoshi Habiro, Motoko Kotani, Susumu Nakae, Taizo Matsuki, Aya Nambu, Shinobu Saijo, Hayato Kotaki, Katsuko Sudo, Akihiko Okahara, Hidetoshi Tanioka, Toshimi Ikuse, Naoto Ishii, Pamela L. Schwartzberg, Ryo Abe, Yoichiro Iwakura

Published in Volume 114, Issue 11

J Clin Invest. 2004; 114(11):1603–1611 doi:10.1172/JCI20742

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Figure 1

Splenocyte and T cell transfer into nu/nu mice. (A) Total splenocytes from IL-1Ra^–/– mice (filled circles, n = 10) induced arthritis, while T cell_depleted splenocytes (open circles, n = 7) did not induce arthritis in nu/nu mice. (B) Arthritic severity score of splenocyte-transferred mice. (C) Purified T cells from spleen and LNs of either arthritic (filled squares, n = 10) or nonarthritic (open squares, n = 8) IL-1Ra^–/– mice induced arthritis in nu/nu mice, while T cells from WT mice (triangles, n = 11) did not. (D) Arthritic severity score of T cell_transferred mice. (E_H) Histology of the ankle joints of WT (E) or IL-1Ra^–/– (F_H) T cell_transferred nu/nu mice. (G) Infiltration of inflammatory cells (indicated by arrowheads). (H) Erosive bone destruction by replacement of bone matrix with fibroblastic cells (indicated by arrowheads). Magnification, ×40 (E and F); ×100 (G and H). (I) Serum IgG (left) and RF (right) levels in WT T cell_ or IL-1Ra^–/– T cell_transferred nu/nu mice. The average in each group is shown as a horizontal bar. WT, WT donors (n = 10); KO+, arthritic-IL-1Ra^–/– donors (n = 10); KO_, nonarthritic IL-1Ra^–/– donors (n = 8). *P < 0.05.