An immunodominant SSX-2–derived epitope recognized by CD4⁺ T cells in association with HLA-DR
J. Clin. Invest. Maha Ayyoub, et al. 113:1225 doi:10.1172/JCI20667 [Go to this article.]

Figure 6
SSX-2–specific CD4⁺ T cells efficiently recognize the SSX-2 native antigen upon processing and presentation by professional APCs. Processing and presentation of SSX-2 antigen by DCs after incubation with recombinant SSX-2 protein (or NY-ESO-1 protein as negative control), or with lysates from tumor cell lines SK-MEL-37 (SSX-2–expressing) or NA8-MEL (SSX-2–negative), was assessed by intracellular staining with anti–IFN-γ antibodies (A) or by ELISA measurement of IFN-γ secretion in the culture supernatant (B).