An immunodominant SSX-2–derived epitope recognized by CD4⁺ T cells in association with HLA-DR
J. Clin. Invest. Maha Ayyoub, et al. 113:1225 doi:10.1172/JCI20667 [Go to this article.]

Figure 2
Identification of the active peptide and recognition in the context of HLA-DR. (A) Intracellular IFN-γ secretion by SSX-2–specific CD4⁺ T cells (clone 1B2) was assessed upon stimulation with a pool containing all overlapping peptides spanning the SSX-2 protein or single peptides in the active P4–6 subpool. (B) Peptide recognition was assessed either in the absence or in the presence of anti–HLA-DR, -DP, or -DQ antibodies. (C) The ability of homozygous EBV-transformed cell lines pulsed with serial dilutions of peptide SSX-2_37–58 to stimulate specific CD4⁺ T cells was assessed by ELISA measurement of IFN-γ secretion in the culture supernatant.