An immunodominant SSX-2–derived epitope recognized by CD4⁺ T cells in association with HLA-DR
J. Clin. Invest. Maha Ayyoub, et al. 113:1225 doi:10.1172/JCI20667 [Go to this article.]

Figure 1
Detection of SSX-2–specific CD4⁺ T cells among circulating T lymphocytes from patient LAU 672 after stimulation with SSX-2–loaded autologous DCs. The presence of specific CD4⁺ T cells in the culture from patient LAU 672 was assessed by staining with phycoerythrin-labeled anti-CD4 mAB and intracellular staining with anti–IFN-γ mAb after incubation in the absence of added peptide or after stimulation with a pool containing all overlapping peptides spanning the SSX-2 protein, or subpools containing three peptides each, as indicated. Numbers in the upper right quadrants are the percentage of cytokine-producing cells among CD4⁺ T cells. Results for all subpools are summarized in Table 1. PE, phycoerythrin.