PPARα governs glycerol metabolism
J. Clin. Invest. David Patsouris, et al. 114:94 doi:10.1172/JCI20468 [
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Figure 5PPARα activation decreases plasma and urine glycerol levels. Enzyme activity of GPDH (
A) or glycerol kinase (
B) was determined in liver homogenates of wild-type and
PPARα-null mice after feeding with Wy14643 (
n = 4 per group). Error bars represent SEM. (
C) AQP3 protein was determined by Western blot in the membrane fraction of liver homogenates of wild-type and
PPARα-null mice treated with Wy14643. Equal amounts of protein were loaded. Glycerol was determined in plasma (
D) (
n = 4) and urine (
E) (samples in each group were pooled and determined in duplicate) in wild-type and
PPARα-null mice after feeding with Wy14643. Significant effects were observed by two-way ANOVA for genotype and for Wy14643 treatment (
P < 0.05). (
F) Plasma glycerol levels decreased in atherosclerotic patients after 4-week treatment with micronized fenofibrate (FF) (250 mg/day). (
P < 0.01, paired Student’s
t test) (
G) Correlation between changes in plasma free fatty acids (FFA) and glycerol in atherosclerotic patients treated with fenofibrate.
