Dysbindin-1 is reduced in intrinsic, glutamatergic terminals of the hippocampal formation in schizophrenia
J. Clin. Invest. Konrad Talbot, et al. 113:1353 doi:10.1172/JCI20425 [Go to this article.]

Figure 4
Dysbindin-1 immunohistochemistry of the human HF. (A) Diagram of the HF, showing subdivisions and layers. The DGiml is highlighted in red to indicate a major neuropil locus of dysbindin-1. The subjacent granule cell layer is shown in green. (B) Dysbindin-1 immunoreactivity (ir) with PA3111Ae2 (1:300) in the section diagrammed in A. (C and D) Antibody specificity test showing that dysbindin-1 immunoreactivity (C) can be eliminated by preadsorption of PA3111Ae2 with 10_7 M recombinant dysbindin-1, M10FL (D). (E and F) Dysbindin-1_positive neurons in CA3 (E) and the DGh (F). (G and H) Dysbindin-1 in the DG. The higher-magnification view in H shows dendrites of hilar neurons containing dysbindin penetrating the granule cell layer (yellow arrowheads) and extending into the molecular layer (green arrowheads). Note the dense band of presynaptic dysbindin-1 in DGiml. CA3p, stratum pyramidal of CA3; PrS, presubiculum; S, subiculum. Hippocampal layers: l, stratum lucidum; m, stratum moleculare; o, stratum oriens; p, stratum pyramidal; r, stratum radiatum. Components of the DG: g, granule cell layer; h, hilus; iml, inner molecular layer; oml, outer molecular layer. Scale bars in A and B: 2 mm; in C and D: 200 ∝m; in E_H: 50 ∝m.